We identified more than 2,000 simple sequence repeats that can be developed as functional molecular markers. Quantitative reverse transcription polymerase chain reaction showed that several well-known stress-responsive genes encoding late embryogenesis abundant protein, dehydrin 1, and ice recrystallization inhibition protein were induced dramatically and that genes encoding U-box-domain-containing protein, electron transfer flavoprotein-ubiquinone, and F-box-containing protein were induced by abiotic stressors in a manner conserved with other plant species. The combined results from all three stress conditions indicated differential expression of 3,110 genes. A total of 29,173 unique protein-coding genes were identified based on sequence similarities to known proteins. Total sequence reads generated by pyrosequencing were assembled into 60,765 unigenes (28,177 contigs and 32,588 singletons). antarctica grown under various stress conditions. Here, we present the first de novo assembly of its transcriptome by massive parallel sequencing and its expression profile using D. However, limited genetic research has focused on this species due to the lack of genomic resources. It has been studied as an extremophile that has successfully adapted to marginal land with the harshest environment for terrestrial plants. Lee, Jungeun Noh, Eun Kyeung Choi, Hyung-Seok Shin, Seung Chul Park, Hyun Lee, HyoungseokĪntarctic hairgrass ( Deschampsia antarctica Desv.) is the only natural grass species in the maritime Antarctic. Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. antarctica and unveils the lack of such a feature in D. caespitosa at low and medium temperatures, confirms a high degree of adjustability of the photosynthetic apparatus in D. Comparison of the P/E values, which were higher in D. The photosynthetic water-use efficiency (photosynthesis-to-transpiration ratio, P/E) emerges as an important factor demonstrating presence of mechanisms which facilitate functioning of a plant in non-optimal conditions. antarctica although the values obtained at 8 degrees C were similar in both species. caespitosa reached the maximum photosynthesis rate at a higher temperature than D. caespitosa has poorer performance in these conditions. These results indicate that the photosynthetic apparatus of D. antarctica, irrespective of the incubation temperature. The fluorescence half-rise times were almost always lower in D. caespitosa decreased during 4 d of incubation at 4 degrees C but it remained stable in D. Measurements of chlorophyll fluorescence showed that the photosystem II (PSII) quantum efficiency of D. antarctica) and to consider how the anticipated climate change may affect vitality of these plants. The aim of our work was to assess photosynthetic plasticity of two hairgrass species with different ecological origins (a temperate zone species, Deschampsia caespitosa (L.) Beauv. antarctica).īystrzejewska-Piotrowska, Grazyna Urban, Pawel L Tufted hairgrass ( Deschampsia caespitosa) exhibits a lower photosynthetic plasticity than Antarctic hairgrass (D.
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